The television programme, hosted by the late Cilla Black from 1985 until 2003, was relaunched two years ago following a 14-year hiatus.
However, next month will mark the first time a contestant is given the option to choose from male and female suitors.
"I’m delighted that Blind Date is continuing to be inclusive by giving a platform to those who identify as bisexual," said O'Grady.
"It was a joy to film and I’m sure Cilla would have been proud."
In the episode, which is due to air in May, beauty consultant Jordan will have to choose between model Jesse, master's student Zoe and support worker Lily.
O'Grady was announced as the host of the revived show in March 2017.
Following the announcement, the comedian, who was close friends with Black, expressed his admiration for the programme's legacy.
"Blind Date is an incredibly special family show which brought laughter, tears and joy to TV viewers throughout the land," he said.
Tributes poured in for the beloved television presenter following the news of her passing.